Search results for " Quantitative PCR"

showing 8 items of 8 documents

Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

2021

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…

ABCC1 ATP binding cassette subfamily C member 1IC50 half maximal inhibitory concentrationMultidrug resistancePharmacologyNADPH reduced nicotinamide adenine dinucleotide phosphateF bioavailabilitychemistry.chemical_compoundPCR polymerase chain reaction0302 clinical medicineMDR multidrug resistanceECL electrochemiluminescencet1/2 elimination half-lifeLC–MS liquid chromatography coupled with mass spectrometryN.D. not detectedGeneral Pharmacology Toxicology and PharmaceuticsBBB blood–brain barriermedia_commonATF3 activating transcription factor 30303 health sciencesChemistryABC ATP-binding cassetteNMPA National Medical Products AdministrationPXR pregnane X receptorSDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresisHBSS Hankʹs balanced salt solutionABCB1Combination chemotherapyProdrugMarsdenia tenacissimaCmax peak concentrationPaclitaxelGAPDH glyceraldehyde-3-phosphate dehydrogenase030220 oncology & carcinogenesisBHI brain heart infusionOriginal ArticleAUC0–∞ area under plasma concentration vs. time curveMRT mean residence timeDrugmedia_common.quotation_subjectRM1-950Vd volume of distributionABCB1 ATP binding cassette subfamily B member 1UIC-2 mouse monoclonal ABCB1 antibodyABCG2 ATP binding cassette subfamily G member 2Combination chemotherapyCYP cytochrome P450 isozymePI propidium iodideTEER transepithelial electrical resistance03 medical and health sciencesPBS phosphate buffer salineFBS fetal bovine serumDox doxorubicinIn vivoPOP polyoxypregnanemedicine030304 developmental biologyEVOM epithelial tissue voltohmmeterTmax time for peak concentrationCancerLBE lowest binding energyPE phycoerythrinmedicine.diseaseMultiple drug resistancePolyoxypregnanePapp apparent permeabilityN.A. not applicableCancer cellH&E hematoxylin and eosinMDR1a multidrug resistance protein 1aTherapeutics. PharmacologyqPCR quantitative PCRM. tenacissima Marsdenia tenacissimaCL clearanceSD standard derivationActa Pharmaceutica Sinica B
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Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids.

2009

This paper reports two new quantitative PCR (qPCR) assays, developed in an attempt to improve the detection of bovine piroplasmids. The first of these techniques is a duplex TaqMan assay for the simultaneous diagnosis of Babesia bovis and B. bigemina. This technique is ideal for use in South America where bovids harbour no theilerids. The second technique, which is suitable for the diagnosis of both babesiosis and theileriosis worldwide, involves fluorescence resonance energy transfer (FRET) probes. In FRET assays, Babesia bovis, B. divergens, Babesia sp. (B. major or B. bigemina), Theileria annae and Theileria sp. were all identifiable based on the melting temperatures of their amplified f…

BabesiaPolymerase Chain ReactionSensitivity and Specificitylaw.inventionlawBabesiosisTheileriaTheileriaFluorescence Resonance Energy TransferTaqManmedicineAnimalsHorsesBabesia bigeminaPolymerase chain reactionGeneral VeterinarybiologyReproducibility of ResultsBabesia bovisBabesiosisGeneral MedicineDNA Protozoanbiology.organism_classificationmedicine.diseaseVirologyMolecular biologyBabesiaTheileria Quantitative PCR Molecular diagnostic TaqMan probes FRET probesTheileriasisReal-time polymerase chain reactionBabesiaCattleHorse DiseasesParasitology
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Evidence for shifts in the structure and abundance of the microbial community in a long-term PCB-contaminated soil under bioremediation.

2011

International audience; Although the impact of bioremediation of PCB-contaminated sites on the indigenous microbial community is a key question for soil restoration, it remains poorly understood. Therefore, a small-scale bioremediation assay made of (a) a biostimulation treatment with carvone, soya lecithin and xylose and (b) two bioaugmentation treatments, one with a TSZ7 mixed culture and another with a Rhodococcus sp. Z6 pure strain was set up. Changes in the structure of the global soil microbial community and in the abundances of different taxonomic phyla were monitored using ribosomal intergenic spacer analysis (RISA) and real-time PCR. After an 18-month treatment, the structure of th…

BioaugmentationEnvironmental Engineeringpolychlorinated biphenyls ; bioremediation ; microbial community structure ; quantitative PCR ; ribosomal intergenic spacer analysisengineering[SDV]Life Sciences [q-bio]Health Toxicology and MutagenesisRibosomal Intergenic Spacer analysis010501 environmental sciencesBiologyReal-Time Polymerase Chain Reactioncomplex mixtures01 natural sciencesenvironmentalmicroorganisme du solActinobacteriaBiostimulation03 medical and health sciencesBioremediationbioremediationSoil functionscivilEnvironmental ChemistryRhodococcusSoil Pollutantsribosomal intergenic spacer analysisWaste Management and DisposalEnvironmental Restoration and RemediationSoil Microbiology0105 earth and related environmental sciencesrelation sol microorganisme2. Zero hunger0303 health sciencessciences and ecology030306 microbiologyEcology15. Life on landbiology.organism_classificationPollutionSoil contaminationPolychlorinated BiphenylsBiodegradation EnvironmentalMicrobial population biologymicrobial community structuresoil restorationEnvironmental chemistryquantitative PCR[SDE]Environmental SciencesbacteriaJournal of hazardous materials
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Identification and Detection of Phoma tracheiphila, Causal Agent of Citrus Mal Secco Disease, by Real-Time Polymerase Chain Reaction.

2006

Phoma tracheiphila is the causal agent of a tracheomycotic disease of citrus called mal secco causing the dieback of twigs and branches. This pathogen is of quarantine concern; therefore, fast and reliable protocols are required to detect it promptly. A specific primer pair and a dual-labeled fluorogenic probe were used in a real-time polymerase chain reaction (PCR) with the Cepheid Smart Cycler II System (Transportable Device TD configuration) to detect this fungus in citrus samples. Real-time PCR assay was compared to modified conventional PCR assay. The sensitivity of the former was evaluated by testing P. tracheiphila DNA dilutions, and the minimum amount detectable was about 500 fg, wh…

CitrusSerial dilutionPhoma tracheiphilaSettore AGR/12 - Patologia VegetaleCitrus limonFungusFungi imperfectiPlant ScienceBiologybiology.organism_classificationlaw.inventionMicrobiologyQuantitative PCRRutaceaeReal-time polymerase chain reactionCitrus; diagnostics; quantitative PCRlawdiagnosticsDiagnosticPathogenAgronomy and Crop SciencePolymerase chain reactionPlant disease
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Genetic potential, diversity and activity of an atrazine-degrading community enriched from a herbicide factory effluent

2008

Aims:  To characterize an atrazine-degrading bacterial community enriched from the wastewater of a herbicide factory. Methods and Results:  The community mineralized 81·4 ± 1·9% of [14C-ring]atrazine and 31·0 ± 1·8% of [14C-ethyl]atrazine within 6 days of batch cultivation in mineral salts medium containing atrazine as the sole nitrogen source. Degradation activity of the community towards different chloro- and methylthio-substituted s-triazine compounds was also demonstrated. Restriction analysis of amplified 16S rDNA revealed high diversity of bacterial populations forming the community, with Pseudomonas species dominating in the clone library. Atrazine-degrading genetic potential of the …

DNA BacterialCOMMUNAUTE BACTERIENNEBioaugmentationWASTEWATERLibraryATRAZINEIndustrial WasteBACTERIAL COMMUNITYBIODEGRADATIONQUANTITATIVE PCRBiologyPolymerase Chain ReactionApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compoundBiotransformationPseudomonasRNA Ribosomal 16STRZAtrazineGenetic variabilityFood science030304 developmental biology0303 health sciencesGenetic diversityBacteriaHerbicidesTriazines030306 microbiologybusiness.industryGeneral Medicine16S ribosomal RNAbiology.organism_classification6. Clean waterBiotechnology[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologyatrazine ; biodegradation ; atz ; trz ; bacterial community ; wastewater ; quantitative PCRchemistryATZbusinessBacteriaPlasmidsBiotechnologyJournal of Applied Microbiology
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Comparative multiplex dosage analysis in spinocerebellar ataxia type 2 patients.

2013

We developed a new application of comparative multiplex dosage analysis (CMDA) for evaluation of the ataxin 2 gene. Expansions of the triplet CAG can cause spinocerebellar ataxia type 2 (SCA2), a neurodegenerative disease with an autosomal-dominant mode of inheritance. Molecular diagnosis of SCA2 is routinely based on the use of conventional PCR to detect the CAG expansion. However, PCR does not amplify an allele with an expansion of many triplets (>80), which is typically found in infantile and juvenile forms of SCA2, thus leading to false negatives. We propose the analysis of the ATXN2 gene by CMDA to complement existing methods currently used for the detection of large expansions of the …

Malecongenital hereditary and neonatal diseases and abnormalitiesGenotypeGene DosagePrenatal diagnosisNerve Tissue ProteinsDiseaseAtaxin 2 Spinocerebellar ataxia type 2 Quantitative PCR Autosomal dominant Prenatal diagnosisSettore BIO/13 - Biologia ApplicataGeneticsMedicineHumansSpinocerebellar AtaxiasMultiplexAlleleMolecular BiologyGeneAllelesGeneticsbusiness.industryGeneral Medicinemedicine.diseaseReal-time polymerase chain reactionAtaxinsAtaxinCase-Control StudiesSpinocerebellar ataxiaFemalebusinessTrinucleotide Repeat ExpansionMultiplex Polymerase Chain ReactionGenetics and molecular research : GMR
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The experience of the International Consortium on Acute Promyelocytic Leukemia in monitoring minimal residual disease in acute promyelocytic leukaemia

2016

OncologyAcute promyelocytic leukemiaacute leukaemiamedicine.medical_specialtyacute promyelocytic leukaemiaPROTEÍNAS PROTO-ONCOGÊNICAS03 medical and health sciences0302 clinical medicineInternal medicinemedicineNeoplasmSurvival ratebusiness.industryFollow up studiesHematologymedicine.diseaseMinimal residual diseaseClinical trialLeukemiaPML/RARA; acute leukaemia; acute promyelocytic leukaemia; minimal residual disease; quantitative PCR030220 oncology & carcinogenesisquantitative PCRminimal residual diseaseAcute promyelocytic leukaemiabusinessPML/RARASettore MED/15 - Malattie del Sangue030215 immunology
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Increased serum miR-193a-5p during non-alcoholic fatty liver disease progression: Diagnostic and mechanistic relevance

2022

Background & Aims Serum microRNA (miRNA) levels are known to change in non-alcoholic fatty liver disease (NAFLD) and may serve as useful biomarkers. This study aimed to profile miRNAs comprehensively at all NAFLD stages. Methods We profiled 2,083 serum miRNAs in a discovery cohort (183 cases with NAFLD representing the complete NAFLD spectrum and 10 population controls). miRNA libraries generated by HTG EdgeSeq were sequenced by Illumina NextSeq. Selected serum miRNAs were profiled in 372 additional cases with NAFLD and 15 population controls by quantitative reverse transcriptase PCR. Results Levels of 275 miRNAs differed between cases and population controls. Fewer differences were seen wi…

SCORING SYSTEMCPM counts per millionAUROC area under the receiver operating characteristicRC799-869AST aspartate aminotransferaseMicroRNA; Non-alcoholic fatty liver disease; Biomarker; SequencingTGF-β transforming growth factor-betaGastroenterologySTEATOHEPATITISLiver disease0302 clinical medicineFibrosismiRNA microRNAlogFC log2 fold changeFIBROSISImmunology and AllergySequencing0303 health scienceseducation.field_of_studyNAS NAFLD activity scoremedicine.diagnostic_testFatty liverGastroenterologyGTEx Genotype-Tissue ExpressionMicroRNADiseases of the digestive system. Gastroenterology3. Good healthReal-time polymerase chain reactionBiomarker MicroRNA Non-alcoholic fatty liver disease SequencingLiver biopsyACIDBiomarker (medicine)030211 gastroenterology & hepatologyLife Sciences & BiomedicineResearch ArticleEXPRESSIONmedicine.medical_specialtyNAFLD non-alcoholic fatty liver diseaseNASH non-alcoholic steatohepatitisPopulationGastroenterology and HepatologySAF steatosis–activity–fibrosisVALIDATIONER endoplasmic reticulum03 medical and health sciencescDNA complementary DNAInternal medicineALT alanine aminotransferaseGastroenterologiInternal MedicinemedicineNAFL non-alcoholic fatty liverALGORITHMFIB-4 fibrosis-4education030304 developmental biologyPCA principal component analysisScience & TechnologyGastroenterology & HepatologyHepatologybusiness.industryBiomarkerFC fold changemedicine.diseaseBiomarker; MicroRNA; Non-alcoholic fatty liver disease; Sequencingdigestive system diseasesFLIP fatty liver inhibition of progressionCt cycle thresholdSteatosisqPCR quantitative PCRbusinessNon-alcoholic fatty liver disease
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